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What Are the Major Types of Chromatography?

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Here we'll go through the types of chromatography. But I think it's a good moment to discuss what the different analytical techniques are separated based on.  So, chromatography is a separation based on polarity differences but distillation is something where you separate based on boiling point differences, extraction involves separation based on acidity or basicity and spectroscopy is a way of identifying what kind of compound do you have by looking at what type of light rays it absorbs. If you are thinking about analytical techniques, it's good to know sort of how they work and their general operation but also understand what they separate based on because that can be a very commonly tested topic. Chromatography is all separated based on polarity differences. it separates based on a stationary phase that is rather polar and it has an adsorbent material which is something polar that other things bind with a mobile phase. Which is oftentimes a liquid sometimes a gas but

What is the different method validation of performance characteristics?

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  This article teaches the basic definition and explanation of each performance characteristic. Typical analytical method parameters are specificity/ selectivity, the limit of the detection, limit of quantification, linearity & range, accuracy, precision, robustness/ ruggedness for analytical procedures. Specificity/ selectivity As per the ICH Q2 guideline, specificity is the ability to assist unequivocally the analyte in the presence of components that may be expected to be present. Typically, these might include impurities degradants matrix, etc.  As per the international union of pure and applied chemistry defined selectivity is the extent to which the method can be used to discover particular analytes in mixture or matrices without intervention from other components of similar behavior. Definition of the various document more or less agree with this interpretation. The international union of pure and applied chemistry recommended the term selectivity. While the pharmaceu

An overview of Method validation

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  In this article, we will learn method validation fitness for purpose of the analytical method What is analytical method validation? What is the difference between method validation and verification?  Why is the method validation necessary? When should methods be validated or verified?  How should the method be validated? What is analytical method validation? Different international documents have given the method validation definition like as per,  ICH Q2: the analytical procedure is to exhibit that it is suitable for its intended purpose.  United States of pharmacopeia USP: the process by which it's established by laboratory studies that the performance characteristics of the procedure meet the requirement for the intended analytical application  ISO 9000 : confirmation, through the stipulation of objective proof that the demand of specific intended use for application have been fulfilled  ISO/IEC 17025: confirmation, by examination and provision of object

What is Column Chromatography and What is the Role of Chromatography in Our Daily Life?

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What is Chromatography Chromatography is the most commonly applied method for the separation of substance mixtures into single components. This powerful technique is for example used in quality control of drugs, in clinical research, or trace analysis of harmful substances. Chromatography is essential for analytical chemistry around the world.  Symtera Analytics has a comprehensive range of chromatography products from sample preparation to subsequent analysis. Provides scientists with the best solution for each analytical task. In HPLC we provide powerful solutions featuring pre-packed columns with many different selectivities.  Column Chromatography It is a chromatography technique in which separation is carried out in columns. The column is filled with the stationary phase which can either be silica, alumina, or cellulose. The bottom of the column is packed with cotton or glass wool to support the stationary phase. The column preparation is usually carried out by two m

What Are Common Types of Laboratory Reagents for Different Processes?

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  Laboratory reagents have a multitude of functions when it comes to chemical reactions or processes. The purpose generally is to initiate a chemical reaction between the reactants while not being part of the formation of the product itself. Sometimes reagents are also used to determine if a certain product is produced, they can be of many forms and structures. Some types of reagents are pure substances and some are compounds or mixtures. They usually come in the form of inorganic chemicals and are sometimes made of organic molecular structures. Some reagents can be used for multiple kinds of reactions while others tend to be specific and serve their purpose for a certain reaction only. Following are types of lab reagents that are commonly used in laboratories. Hydrochloric Acid This acid is very corrosive and has many industrial purposes. It is commonly used to make PVC pipes and polyurethane . While in lower concentration this reagent is used in leather processing in househo

What Is the Role of Analytical Reference Standards in the Analysis of Sample in Quality Control Laboratory?

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In this blog, we are going to discuss what's actually going to do in the quality control lab and what kind of analysis is possible? And during analysis why standards are required? What kind of standards you can employ between the analysis? Before diving into deep discussion , the first thing you should know is the type of analysis commonly performed in the quality control laboratory. Type Of Analysis in The Quality Control Laboratory There are two kinds of analysis ·          Qualitative analysis ·          Quantitative analysis Qualitative Analysis Quality analysis when you are characterizing the physical properties of the material or substance. It means you are doing the description test. You are checking the solubility of the material substance. You are identifying the metals by spectroscopy or HPLC. Here you will not get any measurement values. Quantitative Analysis In a quantitative analysis, you will get measurement values like percentage of water content in

What are the types, Definitions, Properties, and Uses of Primary and secondary reference standards?

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  What are Reference Standards? Solutions of exactly known strength are called standard solutions. A standard solution contains a known weight of chemicals in a definite volume of solution. A standard solution is prepared by dissolving an accurately weighed quantity of highly pure substance in a definite volume of solvent. Types of Standard Solutions There are two types of standard solutions first is primary standard and secondary standard. Primary standard Any reagent or chemical which has all these properties is suitable for use as a primary standard: It is extremely pure.  It is highly stable.  It is anhydrous.  It is less hygroscopic.  It has a very high molecular weight.  It can be weighed easily.  It should be ready to use and available .  It should be preferably nontoxic Following is the detail of a few properties Purity: To qualify as being the primary reference standard, a chemical needs to be extremely pure. Preferably, it should have 99.98% of purity. Ther